Yuav ua li cas ntsuas viscosity yog qhov tseem ceeb rau kev ntsuas particle loj

Cov kws tshawb fawb biopharmaceutical ntsib ob qhov teeb meem tseem ceeb hauv kev tau txais qhov loj me me uas siv cov kev daws teeb meem protein: cov qauv me me uas muaj rau kev sim thiab qhov tsis muaj qhov tseeb viscosity qhov tseem ceeb thaum xam diffusion coefficients thaum lub sij hawm dynamic lub teeb scattering ntsuas.

Lub teeb tawg cuam tshuam li cas rau viscosity? Dynamic light scattering yog ib qho txheej txheem siv los ntsuas submicron particle loj thiab qhov loj me.

Qhov tseeb ntawm qhov ntsuas yog nyob ntawm qhov tseeb ntawm cov ntaub ntawv viscosity muab los ntawm tus neeg siv. Rau kev suav, cov kws tshawb fawb feem ntau xav tias cov tshuaj muaj tib yam viscosity raws li qhov tsis muaj (~ 1 mPa-s). Qhov no tuaj yeem yooj yim ua rau muaj qhov yuam kev loj, tshwj xeeb tshaj yog thaum concentration nce.

Thaum lub sij hawm dynamic lub teeb scattering ntsuas, Brownian motion ntawm hais ua rau lub teeb tawg mus rau ntawm ntau yam intensities, uas yog tom qab ntawd soj ntsuam los txiav txim qhov diffusion coefficient ntawm Brownian motion thiab, yog li ntawd, qhov luaj li cas raws li qhov zoo-paub Stokes-Einstein sib npaug. . Lub diffusion coefficient yog ib qho tseem ceeb uas txiav txim siab ceev ntawm Brownian motion, ua viscosity yog ib qho tseem ceeb hauv kev txiav txim siab particle loj. Qhov kev ua yuam kev yog qhov tseem ceeb vim tias 1% yuam kev hauv viscosity ua rau 1% yuam kev hauv qhov loj me. Cov neeg siv khoom feem ntau ntsib qhov yuam kev nruab nrab ntawm 10% ** 30% hauv kev ntsuas.

Yog li ntawd, kev ntsuas qhov tseeb viscosity ua ntej ua qhov ntsuas lub teeb dynamic yuav ua kom zoo dua cov ntaub ntawv ntsuas.

Txawm hais tias qhov tseeb viscosity tuaj yeem suav txog qhov feem pua ​​​​ntawm qhov ua yuam kev hauv kev ntsuas lub teeb tawg, ntau tus tseem ntsib teeb meem piv txwv thaum nws los txog rau kev ntsuas viscosity.