Phase contrast microscopy txhais
Unstained nyob hlwb nqus yuav luag tsis muaj teeb. Kev nqus lub teeb tsis zoo ua rau muaj qhov sib txawv me me hauv cov duab siv sib faib. Qhov no ua rau cov hlwb NW nyuam qhuav los yog tsis pom nyob rau hauv brightfield microscopy. Phase contrast microscopy yog cov txheej txheem kho qhov muag microscopy uas hloov cov theem hloov hauv lub teeb hla dhau cov qauv pob tshab rau hauv cov kev hloov hauv qhov ci hauv daim duab.
Nws yog thawj zaug piav nyob rau hauv 1934 los ntawm Dutch physicist Frits Zernikes.
Cov ntsiab cai ntawm theem sib piv microscopy
Raws li lub teeb hla dhau ntawm cov hlwb, ib theem me me hloov pauv tshwm sim, uas yog pom tsis tau rau tib neeg lub qhov muag. Nyob rau hauv theem contrast microscopy, cov theem no hloov mus rau hauv amplitude hloov, thiab qhov sib txawv ntawm cov duab sib txawv tuaj yeem pom.
Phase contrast microscopy ua haujlwm
Ib feem coherent illumination uas ua los ntawm tungsten-halogen teeb yog qhia los ntawm ib daim iav collector thiab tsom mus rau lub siab annulus (labeled condenser annulus) nyob rau hauv pem hauv ntej focal dav hlau ntawm sub-stage condenser.
Lub wavefront dhau los ntawm qhov chaw annular illuminates tus qauv thiab tsis deflected los yog theem-diffracted thiab ncua los ntawm cov qauv thiab theem gradients tam sim no nyob rau hauv cov qauv.
Lub teeb tsis pom kev thiab tsis pom kev sib sau los ntawm lub hom phiaj lens yog cais los ntawm theem phaj ntawm sab nraub qaum focal dav hlau thiab tsom mus rau cov duab nruab nrab ntawm lub dav hlau, yog li tsim cov theem kawg sib piv cov duab pom hauv lub qhov muag.
